Metabolic Engineering des Methylerythritolphosphat-Wegs durch Metabolic Control Analysis
نویسندگان
چکیده
منابع مشابه
Metabolic engineering of lactic acid bacteria, the combined approach: kinetic modelling, metabolic control and experimental analysis.
Everyone who has ever tried to radically change metabolic fluxes knows that it is often harder to determine which enzymes have to be modified than it is to actually implement these changes. In the more traditional genetic engineering approaches 'bottle-necks' are pinpointed using qualitative, intuitive approaches, but the alleviation of suspected 'rate-limiting' steps has not often been success...
متن کاملMetabolic flux analysis and metabolic engineering of microorganisms.
Recent advances in metabolic flux analysis including genome-scale constraints-based flux analysis and its applications in metabolic engineering are reviewed. Various computational aspects of constraints-based flux analysis including genome-scale stoichiometric models, additional constraints used for the improved accuracy, and several algorithms for identifying the target genes to be manipulated...
متن کاملMetabolic engineering.
Metabolic engineering is the science that combines systematic analysis of metabolic and other pathways with molecular biological techniques to improve cellular properties by designing and implementing rational genetic modifications. As such, metabolic engineering deals with the measurement of metabolic fluxes and elucidation of their control as determinants of metabolic function and cell physio...
متن کاملMetabolic engineering
MetaCyc database http://metacyc.org/ MetaCyc is a database of metabolic pathways containing more than 1400 pathways from more than 1800 organisms. KEGG pathway database http://www.genome.jp/kegg/pathway.html The KEGG pathway database consists of a collection of manually-curated metabolic maps with connections to metabolite, enzyme and organismal data. KEGG also contains tools for reconstructing...
متن کاملGenetic-Metabolic Coupling for Targeted Metabolic Engineering.
Production of chemicals in microbes often employs potent biosynthetic enzymes, which can interact with the microbial native metabolism to affect cell fitness and product yield. However, production optimization largely relies on data collected from wild-type strains in the absence of metabolic perturbations, thus limiting their relevance to specific conditions. Here, we address this issue by cou...
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ژورنال
عنوان ژورنال: Chemie Ingenieur Technik
سال: 2014
ISSN: 0009-286X
DOI: 10.1002/cite.201450410